Police closed a stretch of the A35 Bournemouth Road in Parkstone around 1.30pm on Saturday afternoon after a serious collision at Castle Hill.
The road was shut between Sandecotes Road and Mansfield Road.
The head-on collision was between a blue Renault Clio and a silver Renault Megane.
An occupant of the Clio suffered serious injuries and two other people, one from each vehicle, were also treated.
The closed section of the road included Ashley Cross.
The road was open again by 4pm.
Sgt Stuart Pitman, senior investigating officer, thanked members of the public for their co-operation while the incident was ongoing, and asked for anyone who saw either of the vehicles prior to the incident to call Dorset Police on 101.
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